Anti-GLDC Polyclonal Antibody for WB, IF/ICC, IP, ELISA - P23378

Core Product Specifications and Parameters

Biological Background: GLDC Function and Localization

• GLDC encodes the P protein of the mitochondrial glycine cleavage system, also known as glycine decarboxylase or glycine dehydrogenase.
• The enzyme catalyzes the decarboxylation of glycine, using pyridoxal phosphate as a cofactor to bind the alpha-amino group and release CO2.
• The remaining methylamine moiety is transferred to the lipoamide cofactor of the H protein (GCSH), a key step in glycine degradation.
• GLDC is localized to the mitochondrion and possesses a transit peptide directing its import.
• Post-translational modifications include acetylation, and the protein is subject to proteomics identification studies.
• Mutations in GLDC are linked to disease variants, and three-dimensional structural data are available.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 36-290 of human GLDC; consider that the antibody may recognize both native and denatured forms.
• For Western blotting, a band approximately 113 kDa is expected; validate with appropriate positive controls.
• In immunofluorescence/immunocytochemistry, mitochondrial localization should be observed; co-staining with mitochondrial markers can confirm specificity.
• Cross-reactivity with mouse and rat is reported; validate in the relevant sample system before use.
• Optimal working dilutions should be determined experimentally; consider testing a range for each application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For glycine metabolism and related research, this GLDC antibody is part of our broad portfolio of high-quality metabolic enzyme antibodies. Count on CamelBio for validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to accelerate your assay development.