Anti-GLB1L3 Rabbit Polyclonal Antibody for WB and ELISA - Q8NCI6

Core Product Specifications and Parameters

Biological Background: GLB1L3 Function and Localization

• GLB1L3 encodes beta-galactosidase-1-like protein 3, a glycosidase belonging to the glycosyl hydrolase family.
• It specifically cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans, playing a role in carbohydrate catabolism.
• This enzymatic activity is essential for the turnover and remodeling of glycoconjugates involved in cell signaling and structure.
• The protein is related to the GLB1 gene family, which includes lysosomal beta-galactosidases with established roles in sphingolipid metabolism.
• Alternative splicing generates multiple transcript variants, potentially yielding isoforms with distinct functional properties or subcellular distributions.
• Proteomics studies confirm GLB1L3 expression at the protein level, and it is part of the human reference proteome, highlighting its biological relevance.
• While specific subcellular localization is not documented, glycosidases often localize to lysosomes, endosomes, or are secreted.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a central region (aa 180–290) of human GLB1L3, which is likely to be accessible in both native and denatured conditions; performance in Western blot should be verified with appropriate controls.
• For Western blot, a band around 75 kDa is anticipated, though alternative splicing may produce additional bands; use a reducing gel and validate with knockdown or knockout samples.
• The antibody is reported to cross-react with mouse and rat; however, users should confirm by sequence alignment of the immunogen region across species and validate in the relevant sample system.
• For ELISA applications, the polyclonal antibody can be used for detection or capture, but optimal concentrations and conditions need to be empirically determined.
• Account for the glycosidase nature of GLB1L3 by including protease inhibitors and maintaining appropriate buffer conditions during sample preparation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a streamlined one-stop platform for sourcing IVD raw materials, including this GLB1L3 polyclonal antibody for glycosidase and ganglioside research. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents, with the capability to address rare target sourcing needs. Partner with CamelBio to secure reliable supply and accelerate your assay development projects.