Anti-GKN1 Polyclonal Antibody for WB, ELISA - Q9NS71

Core Product Specifications and Parameters

Biological Background: Gastrokine-1 Function and Localization

• Gastrokine-1 (GKN1), also known as AMP-18 or foveolin, is a secreted protein specifically expressed in the stomach epithelium.
• It exerts mitogenic activity and is believed to play a role in maintaining the integrity of the gastric mucosal lining.
• Subcellular localization includes cytoplasmic granules, the Golgi apparatus, and secretion into the extracellular space, supporting its processing and release.
• At the protein level, GKN1 is detected in normal stomach tissue but is absent in gastric cancer tissues and cell lines, suggesting a potential role in mucosal homeostasis that is lost in malignancy.
• The protein features a signal peptide, contains disulfide bonds, and has been identified through proteomics studies, classifying it as a secreted and reference proteome member.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 25–185 of human GKN1, corresponding to the mature protein form; consider that this antibody may recognize both intracellular precursor and secreted mature protein.
• For Western blot, stomach tissue lysates should serve as a suitable positive control, with an expected band near 20 kDa; note that post‑translational modifications might result in slight molecular weight shifts.
• Since endogenous GKN1 is not expressed in gastric cancer cell lines, validate the antibody using normal gastric mucosa samples or primary stomach tissue to ensure relevant detection.
• Cross‑reactivity with mouse enables possible use in murine gastrointestinal models; verify specificity by testing mouse stomach extracts and comparing to human samples.
• When developing ELISA applications, pair this polyclonal antibody with a suitable capture reagent and optimize conditions using recombinant GKN1 or native protein from stomach homogenates.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research institutes one‑stop access to high‑quality IVD raw materials, including validated antibodies such as this anti‑GKN1 polyclonal antibody. Our comprehensive supply capabilities cover validated pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare‑target sourcing to accelerate your gastrointestinal research and assay development projects. Trust CamelBio to bridge the gap from concept to clinic with reliable, scalable raw‑material solutions.