Anti-GJD2 Rabbit pAb - Q9UKL4

Core Product Specifications and Parameters

Biological Background: GJD2 Function and Localization

• Gap junction delta-2 protein (GJD2), also known as connexin-36 (Cx36) or gap junction alpha-9 protein (GJA9), is a member of the connexin family that forms intercellular channels.
• GJD2 assembles into hexameric connexons (hemichannels) that dock between adjacent cells, forming gap junction plaques that allow direct diffusion of ions and small molecules (<1 kDa) between cells.
• The human GJD2 gene (ID: 57369; alias GJA9) encodes this multi-pass transmembrane protein, which is central to electrical and metabolic coupling in excitable tissues.
• Subcellularly, GJD2 is localized to the cell membrane and specifically enriched at gap junctions, the specialized cell-cell contact sites.
• The protein is highly and preferentially expressed in neurons, where it contributes to neuronal synchronization, network oscillations, and sensory processing.
• Key structural features include four transmembrane helices, intracellular N- and C-termini, and extracellular loops that mediate connexon docking; it is also annotated with a known 3D structure.
• UniProt keyword annotations include reference proteome, proteomics identification, and confirmed transmembrane helix topology, underscoring its experimental tractability.

Experimental Guidance and Technical Tips

• For Western blotting, a band around 36 kDa is expected under reducing conditions; note that connexin oligomerization may lead to higher molecular weight species.
• Given the immunogen corresponds to a cytoplasmic loop region (amino acids 99–197), the antibody is likely to recognize intracellular epitopes; consider membrane protein enrichment or increased detergent for optimal solubilization.
• Validate cross-reactivity in your specific model system (human, mouse, rat) and with relevant positive controls such as brain or neuronal tissue lysates.
• For ELISA applications, assess the antibody as a capture or detection reagent; pairing with a monoclonal anti-GJD2 antibody may improve sandwich assay specificity.
• As a polyclonal antibody, perform lot-to-lot consistency checks if used in quantitative or regulated assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic developers and researchers with reliable access to IVD raw materials, including validated polyclonal antibodies like this anti-GJD2 product. Whether you are developing assays for gap junction targets in neuroscience or exploring connexin-36 as a biomarker, our platform streamlines procurement of optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target materials. Partner with us to bridge your project from concept to clinic.