Anti-GJB3 Polyclonal Antibody for WB, ELISA - O75712

Core Product Specifications and Parameters

Biological Background: GJB3 Function and Localization

• GJB3, also known as Connexin-31 (Cx31), is a member of the gap junction beta family and functions as a subunit of gap junction channels.
• Gap junctions composed of connexin proteins facilitate direct intercellular communication by allowing the passage of ions, metabolites, and small molecules (low molecular weight) between adjacent cells.
• GJB3 is localized to the cell membrane and specifically enriched at gap junction plaques, where it forms hexameric connexons that dock with those of neighboring cells.
• Mutations in GJB3 are associated with autosomal dominant non-syndromic sensorineural deafness (DFNA2) and erythrokeratodermia variabilis (EKV), highlighting its critical role in hearing and skin integrity.
• The protein contains four transmembrane domains, with both N- and C-termini located in the cytoplasm, and is post-translationally modified by phosphorylation, which regulates channel assembly and function.
• GJB3 is expressed in various tissues, including the cochlea, skin, and placenta, consistent with its involvement in tissue-specific functions.

Experimental Guidance and Technical Tips

• For Western blotting, a band corresponding to the predicted molecular weight of 31 kDa should be detected. Consider using positive control lysates from human, mouse, or rat cell lines expressing GJB3.
• The immunogen is a synthetic peptide from the internal region (aa 100-200), suggesting reactivity with total GJB3 protein. Cross-reactivity with other connexin isoforms has not been determined; verify specificity using blocking peptides or knockout models if needed.
• For ELISA applications, this antibody can be used to capture or detect GJB3; optimize coating concentration and detection system according to your assay format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials for gap junction biology and genetic deafness research, including validated antibody pairs, recombinant proteins, and ancillary reagents. With our one-stop platform, diagnostic manufacturers and laboratories can access optimized polyclonal and monoclonal antibodies, along with technical consulting, to accelerate assay development from concept to clinic. Contact our team for bulk quantities or custom sourcing of rare targets like connexins.