Anti-GJA8 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P48165

Core Product Specifications and Parameters

Biological Background: GJA8 Function and Localization

• GJA8 encodes gap junction alpha-8 protein, also known as Connexin-50 (Cx50) and lens fiber protein MP70.
• It functions as a structural component of eye lens gap junctions, forming intercellular channels that allow direct diffusion of small molecules and ions between adjacent cells. Related references: PMID:18006672, PMID:19756179
• Gap junctions are dodecameric channels formed by the docking of two hexameric hemichannels, one from each adjoining cell membrane.
• GJA8 is specifically expressed in the eye lens tissue.
• Subcellularly, GJA8 localizes to the cell membrane and is enriched at gap junctions.
• Keywords associated with GJA8 include cataract and disease variant, highlighting its clinical relevance in lens pathologies; it is a multi-pass transmembrane protein with disulfide bonds.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 234-433 of human GJA8, which covers part of the cytoplasmic tail; this region may offer specificity against other connexins.
• For Western blot, expect a band near 48 kDa; consider that post-translational modifications or aggregation could shift migration.
• Validate antibody performance in relevant sample systems such as lens tissue extracts or GJA8-expressing cell lines.
• For IF/ICC, anticipate membrane and gap junction punctate staining; permeabilization may be required.
• Cross-reactivity with mouse and rat enables use in rodent models of lens biology and cataract.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with one-stop access to IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. This anti-GJA8 antibody supports investigations into gap junction biology and cataract-related research, and CamelBio can assist with sourcing rare targets and scaling up raw material supplies from concept to clinic.