Anti-GJA5/CX40 Rabbit Polyclonal Antibody for IF/ICC, ELISA - P36382

Core Product Specifications and Parameters

Biological Background: GJA5 (Connexin-40) Function and Localization

• GJA5 encodes gap junction alpha-5 protein, commonly referred to as Connexin-40 (Cx40) or ATFB11.
• Functions as a subunit of gap junction channels that allow the direct diffusion of low molecular weight molecules between adjacent cells.
• Each gap junction channel is formed by the docking of two hexameric connexons, with GJA5 being a member of the connexin gene family.
• Localizes primarily to the cell membrane at gap junction plaques, mediating intercellular communication.
• Undergoes post-translational phosphorylation, as indicated by the presence of phosphoprotein annotation.
• Genetic variants in GJA5 have been associated with atrial fibrillation and cardiomyopathy, highlighting its role in cardiac electrophysiology.
• The mature protein has a molecular weight of approximately 40 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 229-358), suggesting the antibody likely recognizes an intracellular epitope; consider using permeabilization (e.g., Triton X-100) for IF/ICC.
• Validated for immunofluorescence/immunocytochemistry (IF/ICC) and ELISA; optimize dilutions and incubation conditions in your specific assay system.
• Reported cross-reactivity is with human samples; verify performance in other species if needed using appropriate controls.
• For gap junction studies, ensure proper sample preparation to preserve membrane structures and confirm specificity with knockout or knockdown controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports your diagnostic and research workflows with access to a broad portfolio of IVD raw materials, including this anti-GJA5 rabbit polyclonal antibody for cardiovascular and cell communication studies. We offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target raw-material sourcing to meet your development needs. Partner with CamelBio to streamline your path from concept to clinic.