Anti-GJA5 Polyclonal Antibody for WB, ELISA - P36382

Core Product Specifications and Parameters

Biological Background: GJA5 Function and Localization

• GJA5 encodes gap junction alpha-5 protein, also known as Connexin-40 (Cx40).
• Functions as a structural component of gap junctions, which are clusters of intercellular channels that permit the direct exchange of ions, metabolites, and small signaling molecules between adjacent cells.
• These channels are formed by connexons, each composed of six connexin subunits, which dock across the intercellular space to create a hydrophilic pore.
• Localizes to the cell membrane and is enriched at gap junction plaques.
• Plays a critical role in cardiac conduction and vascular homeostasis; mutations in GJA5 are associated with atrial fibrillation and cardiomyopathy.
• Undergoes phosphorylation, which regulates channel assembly, trafficking, and gating properties.
• Contains four transmembrane domains with intracellular N- and C-termini, typical of the connexin family.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal cytoplasmic domain (aa 229-358) of human GJA5, which may confer specificity for the Cx40 isoform; cross-reactivity with other connexins should be assessed in your experimental system.
• For Western blot, a band of approximately 40 kDa is expected; consider using positive controls such as cardiac tissue lysates or HEK293 cells transfected with GJA5.
• For ELISA, immobilize purified recombinant GJA5 protein or the immunogen peptide to validate antibody binding; check for plate-coating and blocking conditions that minimize background.
• As a polyclonal antibody, it may recognize multiple epitopes; pre-adsorption against the immunogen can reduce non-specific binding if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This GJA5 antibody supports research into gap junction biology and cardiovascular disease diagnostics. We offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and assistance with rare target raw-material sourcing to streamline your assay development.