Anti-GJA4 Polyclonal Antibody for WB, ELISA - P35212

Core Product Specifications and Parameters

Biological Background: GJA4 Function and Localization

• GJA4 encodes the gap junction alpha-4 protein, also known as Connexin-37 (Cx37), a member of the connexin family of transmembrane proteins.
• It forms intercellular channels (gap junctions) that permit the passive diffusion of low molecular weight molecules, such as ions, metabolites, and second messengers, between adjacent cells.
• Gap junctions are assembled from connexons, each comprising a hexamer of connexin subunits, and are critical for coordinated cellular activities in tissues.
• Subcellular localization includes the cell membrane, specifically at cell–cell contact sites forming gap junction plaques.
• Tissue expression is reported in multiple organs, including heart, uterus, ovary, and blood vessel endothelium, suggesting roles in cardiovascular and reproductive physiology.
• Key structural features include four transmembrane helices, intracellular N- and C-termini, and two extracellular loops, consistent with canonical connexin topology.
• The protein has been identified through proteomics and is part of the reference proteome for human.

Experimental Guidance and Technical Tips

• The immunogen spans a C‑terminal cytoplasmic region (amino acids 229‑333 of the human GJA4 protein), which is expected to generate antibodies recognizing the full‑length protein; this region may show low cross‑reactivity with other connexins due to sequence divergence.
• For Western blotting, a band around 37 kDa (calculated molecular weight 37.4 kDa) may be detected; however, post‑translational modifications or aggregation can yield additional bands, so inclusion of appropriate controls is recommended.
• ELISA can be used for titer determination or detection of native/denatured protein; consider employing peptide competition to confirm specificity.
• Cross‑reactivity with rat indicates conservation of the epitope; validation in the intended species and tissue system is advised.
• For gap junction research, consider testing in cell lines endogenously expressing GJA4, such as HUVECs or ovarian granulosa cells, to ensure physiological relevance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your go‑to partner for IVD raw material procurement, offering a streamlined supply of validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare‑target sourcing services. For researchers focusing on cell communication, vascular biology, or reproductive health, CamelBio’s expertise in delivering high‑quality raw materials—including connexin‑targeted antibodies and related assay components—can accelerate your development from concept to clinic.