Anti-GITR/TNFRSF18 Rabbit Polyclonal Antibody for WB, ELISA - Q9Y5U5

Core Product Specifications and Parameters

Biological Background: GITR/TNFRSF18 Function and Localization

• GITR (TNFRSF18) is a member of the tumor necrosis factor receptor superfamily, also known as AITR or CD357.
• It functions as a receptor for TNFSF18 (GITR ligand) and is implicated in T-cell-mediated immune responses.
• Involved in interactions between activated T-lymphocytes and endothelial cells, and in regulating T-cell receptor-mediated cell death.
• Mediates NF-kappa-B activation through the TRAF2/NIK signaling pathway.
• Localized to the cell membrane, but a secreted isoform may also exist, as indicated by alternative splicing.
• Expressed predominantly in lymph node, peripheral blood leukocytes, and weakly in spleen.
• Contains characteristic TNF receptor features including disulfide bond-rich repeats and a transmembrane helix; is a glycoprotein.

Experimental Guidance and Technical Tips

• The immunogen covers the extracellular domain (aa 26-255), which may facilitate recognition of both membrane-bound and soluble forms.
• For Western blotting, consider using lysates from lymph node or leukocyte cell lines where GITR expression is reported.
• Given the reported mouse cross-reactivity, validate antibody specificity in the relevant species and sample system before critical experiments.
• For ELISA, consider an indirect format with recombinant GITR protein as the capture target.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio accelerates your diagnostic development by providing streamlined access to high-quality IVD raw materials, including this GITR/TNFRSF18 antibody for T-cell biology and immuno-oncology research. Our comprehensive portfolio spans validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing of rare targets—supporting every stage from concept to clinic.