Anti-GITR Ligand/TNFSF18 Rabbit Polyclonal Antibody for WB, ELISA - Q9UNG2

Core Product Specifications and Parameters

Biological Background: Tumor necrosis factor ligand superfamily member 18 Function and Localization

• Member of the tumor necrosis factor (TNF) ligand superfamily, also known as GITR ligand (GITRL), AITRL, or TL6.
• Functions as a cytokine that binds to the receptor TNFRSF18 (GITR), acting as a costimulatory molecule to lower the threshold for T-cell activation and promote T-cell proliferation.
• Plays a key role in mediating interactions between activated T lymphocytes and endothelial cells, enhancing leukocyte adhesion through upregulation of VCAM1 and ICAM1. Related references: PMID:23892569
• Mediates activation of NF-kappa-B signaling and triggers increased phosphorylation of STAT1. Related references: PMID:23892569
• Regulates monocyte migration from the splenic reservoir to sites of inflammation.
• Localizes to the cell membrane as a single-pass type II membrane protein.
• Highly expressed in the small intestine, ovary, testis, kidney, and endothelial cells.
• Contains a disulfide bond and is subject to glycosylation; predicted molecular weight of approximately 20.3 kDa.

Experimental Guidance and Technical Tips

• For Western blotting, a band around 20–25 kDa is anticipated, considering potential glycosylation. Validate the antibody in your relevant sample system (human, mouse, or rat) and optimize blocking/detection conditions.
• For ELISA, consider using the recombinant immunogen (aa 52–177) as a coating antigen or pair with a matched detection antibody. Establish optimal dilutions for both capture and detection steps.
• As a polyclonal antibody, it may exhibit batch-to-batch variation; we recommend titrating the antibody for your specific application and including appropriate positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers and research institutions from concept to clinic. This anti-GITR Ligand/TNFSF18 polyclonal antibody is part of our extensive portfolio tailored for T-cell activation and immunotherapy research. We offer bulk supply, validated antibody pairs, optimized monoclonal/polyclonal reagents, and access to rare target materials to streamline your assay development.