Anti-GIT2 Rabbit Polyclonal Antibody for WB, ELISA - Q14161

Core Product Specifications and Parameters

Biological Background: GIT2 Function and Localization

• GIT2, also known as CAT-2, CAT2, PKL, or GRK-interacting protein 2, is a member of the GIT family of ARF GTPase-activating proteins.
• Functions as a GTPase-activating protein (GAP) for ADP-ribosylation factor (ARF) proteins, including ARF1, negatively regulating their activity.
• Acts as a scaffold linking G protein-coupled receptor kinases (GRKs) and p21-activated kinases (PAKs) to focal adhesion complexes.
• Contains ankyrin repeats and a coiled-coil domain that mediate protein-protein interactions, as well as a zinc-finger motif.
• Alternative splicing produces multiple isoforms; annotated as a phosphoprotein in several proteomics studies.
• Plays roles in cell adhesion, migration, and receptor internalization through modulation of ARF-dependent membrane trafficking.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a central region (aa 450-510) and may detect multiple GIT2 isoforms; verify band patterns in relevant cell lysates.
• For Western blot, the predicted molecular weight is ~85 kDa; use positive controls such as HeLa or HEK293 whole-cell extracts.
• In ELISA, titrate the antibody against the immunogen or recombinant GIT2 to determine the optimal working concentration.
• Confirm specificity through peptide blocking or siRNA-mediated knockdown experiments in your assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio acts as a comprehensive sourcing partner for IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For research on ARF signaling, GPCR biology, or intracellular trafficking, we offer one-stop access to GIT2-targeting reagents and can assist with rare target raw-material procurement to accelerate your assay development.