Anti-GIRK2 (KCNJ6) Rabbit Monoclonal Antibody for WB, IF/ICC, IF-P, ELISA - P48051

Core Product Specifications and Parameters

Biological Background: GIRK2 Function and Localization

• G protein-activated inward rectifier potassium channel 2 (GIRK2), also known as KCNJ6 or KATP-2, is a member of the inwardly rectifying potassium channel family.
• It mediates the flow of potassium ions into cells, with voltage dependence regulated by extracellular potassium concentration; inward rectification is primarily due to blockage of outward current by intracellular magnesium.
• GIRK2 is implicated in the regulation of insulin secretion by glucose and neurotransmitters through G protein-coupled receptors, linking it to metabolic processes.
• The protein is most abundantly expressed in the cerebellum, and to a lesser extent in pancreatic islets and exocrine pancreas, consistent with roles in neuronal signaling and hormone secretion.
• Immunofluorescence and biochemical studies localize GIRK2 primarily to the plasma membrane, where it functions as an ion channel.
• Mutations in KCNJ6 are associated with congenital generalized lipodystrophy and intellectual disability, emphasizing its clinical relevance.
• Post-translational modifications include phosphorylation, which may regulate channel activity.
• The channel is also classified under the inward rectifier potassium channel subfamily J, member 6, with a predicted molecular weight of approximately 48 kDa.

Experimental Guidance and Technical Tips

• This rabbit monoclonal antibody is suitable for Western blot (WB), immunofluorescence (IF/ICC, IF-P), and ELISA. Performance should be validated in the relevant sample system and experimental context.
• The immunogen is a synthetic peptide from the N-terminal region (amino acids 1–100); for Western blotting, a band near 48 kDa is expected, corresponding to the full-length GIRK2 protein.
• Because the antibody cross-reacts with human and rat, it can be applied to tissues or cell lines from these species; consider optimizing dilutions and controls for each application.
• For immunofluorescence, expect membrane-localized staining. Co-staining with organelle markers can help confirm subcellular distribution.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with a one-stop platform for IVD raw materials. Our portfolio includes validated antibodies like this anti-GIRK2 monoclonal antibody, supporting research into ion channel-related diseases and metabolic disorders. We also offer bulk reagents, customized sourcing, and technical support to accelerate your assay development.