Anti-GIPR Polyclonal Antibody for WB, IHC-P, ELISA - P48546

Core Product Specifications and Parameters

Biological Background: GIPR Function and Localization

• GIPR (Gastric inhibitory polypeptide receptor, also known as glucose-dependent insulinotropic polypeptide receptor) is a receptor for the incretin hormone GIP, playing a key role in insulin secretion.
• The receptor belongs to the G protein-coupled receptor (GPCR) family and mediates its effects via G proteins that activate adenylyl cyclase, leading to increased cAMP levels.
• Encoded by the GIPR gene (GeneID: 2696) in humans, the protein is subject to alternative splicing, potentially yielding multiple isoforms.
• GIPR is located on the cell membrane, with a typical seven-transmembrane domain structure characteristic of GPCRs.
• Post-translational modifications include disulfide bonds and glycosylation, which are essential for proper folding and function.
• Keywords from UniProt highlight its role as a receptor, transducer, and membrane protein, with a signal peptide for membrane targeting.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 22-138 of human GIPR, which lies within the N-terminal extracellular domain. This region is likely exposed and may be suitable for detecting the receptor in native conformation.
• For western blot (WB), consider a standard protocol; the predicted band is approximately 53 kDa, but additional bands may appear due to glycosylation or alternative splicing.
• In immunohistochemistry (IHC-P), membrane staining is expected; include appropriate controls and validate in tissues known to express GIPR, such as pancreas and adipose tissue.
• For ELISA, this antibody may serve as a capture or detection reagent; pairing with a compatible antibody is advisable. Validate sensitivity and specificity in the relevant sample matrix.
• Cross-reactivity with mouse and rat GIPR is reported; however, sequence homology should be verified to ensure conserved epitope recognition.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted one-stop partner for IVD raw materials, empowering diagnostic manufacturers and research institutions with seamless access to high-quality reagents. This GIPR polyclonal antibody supports research in metabolic disorders, diabetes, and obesity, and can be complemented by our extensive catalog of validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For rare or emerging targets, our sourcing and technical services streamline your path from concept to clinic.