Anti-GIPC2 Polyclonal Antibody for WB and ELISA - Q8TF65

Core Product Specifications and Parameters

Biological Background: GIPC2 Function and Localization

• GIPC2 is a PDZ domain-containing protein, members of which typically mediate protein-protein interactions in signaling pathways.
• The gene encoding this protein is also known as SEMCAP2.
• Subcellularly, GIPC2 localizes primarily to the cytoplasm.
• Tissue expression profiling shows highest levels in ascending colon, moderate levels in adult kidney, low levels in pancreas, and very low levels in liver.
• Expression is down-regulated in several primary tumor types, including kidney, colon, and rectal tumors, suggesting a potential tumor-suppressive role.
• The predicted molecular weight is 34 kDa, consistent with proteomics identification.
• A three-dimensional structure has been determined, facilitating understanding of its interaction interfaces.

Experimental Guidance and Technical Tips

• The immunogen covers the full-length human GIPC2 protein (amino acids 1–315), which may enable recognition of both native and denatured forms in Western blotting. For WB, consider using whole cell lysates from human colon or kidney cell lines and look for a band around 34 kDa.
• In ELISA applications, use the recombinant immunogen as a positive control to assess specificity and sensitivity.
• Given the tissue expression pattern, validate antibody performance in relevant normal and tumor tissue samples, such as colon and kidney, to confirm cross-reactivity and down-regulation findings.
• For immunohistochemistry or other applications not listed, optimize experimentally as specificity may differ from WB/ELISA.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with integrated access to IVD raw materials, supporting projects from concept to clinical application. This anti-GIPC2 polyclonal antibody complements cancer biomarker research, particularly in colorectal and renal tumor suppression pathways. Through CamelBio, customers can source validated antibody pairs, bulk ancillary reagents, and customized raw-material solutions for rare targets like GIPC2, streamlining procurement for assay development.