Anti-GINS2 Rabbit Polyclonal Antibody for WB and ELISA - Q9Y248

Core Product Specifications and Parameters

Biological Background: GINS2 Function and Localization

• GINS2 (PSF2) is a subunit of the GINS complex (Go-Ichi-Ni-San), essential for DNA replication initiation and fork progression. It is a core component of the CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA. Related references: PMID:17417653 PMID:32453425 PMID:34694004 PMID:34700328
• The GINS complex comprises four subunits (Sld5, Psf1, Psf2, Psf3) and is conserved across eukaryotes.
• GINS2 localizes to the nucleus and associates with chromosomes during replication.
• Post-translational modifications of GINS2 include acetylation, phosphorylation, and ubiquitination, suggesting regulatory roles in replication control.
• The protein has a predicted molecular weight of 21 kDa and has been structurally characterized (3D-structure available).
• Identified as a reference proteome component in proteomics studies.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (amino acids 1–185) of human GINS2; for WB, a band at ~21 kDa is anticipated. Validate specificity using knockdown or overexpression samples.
• For ELISA applications, the antibody may serve as a capture or detection reagent; consider pairing with another anti-GINS2 antibody or the target protein.
• Optimal dilutions should be determined empirically by the end-user in the relevant sample system.
• The antibody shows reactivity with human GINS2; cross-reactivity with other species has not been tested.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic developers, research laboratories, and IVD manufacturers streamlined access to critical raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For projects targeting DNA replication or helicase complexes, we can source rare target raw materials such as anti-GINS2 antibodies, supporting your workflow from concept to clinic with tailored procurement solutions.