Anti-GIMAP5 Rabbit Polyclonal Antibody for WB, ELISA - Q96F15

Core Product Specifications and Parameters

Biological Background: GIMAP5 Function and Localization

• GIMAP5 (GTPase IMAP family member 5) is a member of the immune-associated nucleotide-binding protein (IAN) family, also known as IAN-5, hIAN5, and IMAP3. It is a GTP-binding protein with a transmembrane domain.
• Plays a critical role in T lymphocyte development, particularly in the optimal generation of CD4/CD8 double-positive thymocytes, as suggested by similarity data.
• Acts as an inhibitor of glycogen synthase kinase-3 alpha (GSK3A) by sequestering GSK3A in cytoplasmic vesicles, which impairs its nuclear translocation and downstream transcriptional programs, thereby regulating the DNA damage response during T cell proliferation. Related references: PMID:29382851
• Required for the survival of peripheral T cells, natural killer (NK) cells, and NKT-cell development, and is essential for the maintenance of normal liver function, based on similarity evidence.
• May promote the survival of mature T lymphocytes upon cytokine withdrawal and regulate calcium homeostasis by modulating lysosomal Ca²⁺ stores.
• Localizes to the membranes of lysosomes, endosomes, and multivesicular bodies, indicating a role in intracellular membrane trafficking.
• Widely expressed with high levels in lymph node and spleen; highly expressed in T lymphocytes (CD4⁺ and CD8⁺ T-cells) and monocytes, but very low in B-lymphocytes. Related references: PMID:14724691

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 190-270 of human GIMAP5; this region may be conserved across splicing variants. For WB, validate in the relevant cell type or tissue known to express GIMAP5.
• For western blotting, a band around 35 kDa is expected in human, mouse, and rat samples. Consider using a positive control such as Jurkat T-cell lysate.
• In ELISA, assess coating antigen concentrations and detection antibody dilutions for optimal performance; the antibody may be used for capture or detection in sandwich ELISAs targeting GIMAP5.
• Since the antibody is polyclonal, it may recognize multiple epitopes; confirm specificity via knockdown or overexpression experiments if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive IVD raw material solutions, supporting your GIMAP5-related research and diagnostic development with validated antibody pairs, bulk polyclonal antibodies, and ancillary reagents. Our one-stop platform streamlines procurement for T-cell biology and immune function studies, ensuring consistent quality from discovery to clinical applications. Whether you need rare-target reagents or scalable antibody production, CamelBio bridges your sourcing needs with expert consultation.