Anti-GIF Rabbit Polyclonal Antibody for WB, ELISA - P27352

Core Product Specifications and Parameters

Biological Background: GIF Function and Localization

• The protein encoded by the CBLIF (GIF) gene is gastric intrinsic factor (IF), also known as cobalamin binding intrinsic factor, essential for vitamin B12 (cobalamin) absorption.
• GIF is a glycoprotein secreted by gastric mucosal cells, predominantly parietal cells, and is produced as a precursor with a signal peptide.
• Functionally, GIF binds free cobalamin in the stomach; after proteolytic cleavage in the duodenum, the stable GIF-cobalamin complex interacts with the cubilin (CUBN) receptor in the ileum, facilitating receptor-mediated endocytosis for vitamin B12 uptake.
• The GIF gene undergoes alternative splicing, yielding multiple isoforms; disease-associated variants in CBLIF cause intrinsic factor deficiency (IFD), leading to pernicious anemia due to impaired B12 absorption.
• GIF is a phosphoprotein with multiple disulfide bonds critical for its structure and function, and it belongs to a group of proteins involved in cobalt and ion transport.
• Tissue expression is highly specific to the gastric mucosa, consistent with its role in gastrointestinal vitamin absorption.
• Keywords associated with this protein include disease variant, glycoprotein, secreted, signal, and transport, highlighting its clinical and functional significance.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant protein encompassing amino acids 19-417 of human GIF, corresponding to the mature protein without the signal peptide, which suggests the antibody can recognize the endogenous secreted form.
• For Western blot, consider comparing reducing and non-reducing SDS-PAGE conditions, as GIF contains multiple disulfide bonds that may influence electrophoretic mobility.
• Given the tissue-specific expression in gastric mucosa, validate the antibody in stomach lysates from human, mouse, or rat, and consider using a negative tissue like liver for specificity control.
• In ELISA, this polyclonal antibody can be explored as a capture or detection reagent; for sandwich ELISA, pairing with a monoclonal anti-GIF antibody is recommended to enhance specificity.
• Since GIF is a secreted protein, when analyzing cell or tissue culture supernatants, concentration steps may be necessary to detect endogenous levels by WB or ELISA.
• Because cross-reactivity is reported for mouse and rat, verify epitope conservation and assay performance in these species using appropriate positive controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic developers, research labs, and institutes with streamlined access to IVD raw materials, technical services, and consulting, supporting projects from concept to commercialization. For studies on vitamin B12 metabolism, pernicious anemia, or gastrointestinal diagnostics, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and tailored sourcing for rare targets like GIF. Rely on CamelBio as your single touchpoint for accelerating assay development with dependable raw material supply.