Anti-Giantin Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q14789

Core Product Specifications and Parameters

Biological Background: Giantin Function and Localization

• Giantin, encoded by the GOLGB1 gene (GeneID: 2804), is a large Golgi membrane protein with a calculated molecular weight of ~376 kDa; it is also known as GCP372, Macrogolgin, and GOLIM1.
• It belongs to the golgin family, characterized by extensive coiled-coil domains that facilitate tethering and structural organization within the Golgi apparatus.
• Giantin functions as a structural component, participating in the formation of intercisternal cross-bridges that maintain Golgi stack integrity and architecture.
• It localizes exclusively to the Golgi apparatus membrane, anchored via a single C-terminal transmembrane helix.
• Post-translational modifications include acetylation and phosphorylation, which may regulate its function and protein-protein interactions.
• Alternative splicing generates multiple isoforms, potentially contributing to functional diversity in different cellular contexts.
• As a proteomics-identified reference protein, Giantin is widely studied in Golgi organization and vesicular trafficking.

Experimental Guidance and Technical Tips

• The immunogen spans a region within the central coiled-coil domain of human Giantin (aa 1123–1302), likely recognizing a conserved epitope present in major isoforms.
• For Western blotting, consider using low-percentage gels and extended transfer times due to the large protein size (~376 kDa); a high-molecular-weight marker is essential.
• In immunofluorescence/ICC, expect a characteristic perinuclear Golgi-like staining pattern; co-staining with known Golgi markers (e.g., GM130) is recommended to confirm specificity.
• For ELISA, this antibody can be used to detect recombinant Giantin fragments or capture the protein from cell lysates; assay optimization is advised for sensitive detection.
• Cross-reactivity is reported for human; if using with other species, validate experimentally to ensure specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research institutes, and laboratories with a one-stop supply chain for IVD raw materials, including validated antibodies like this anti-Giantin polyclonal antibody. Our portfolio covers a wide range of targets for Golgi biology and cellular trafficking studies, with dedicated support for sourcing bulk reagents, ancillary materials, and rare target antibodies. Partner with CamelBio to streamline your procurement from concept to clinic.