Anti-GHRL Polyclonal Antibody for WB and ELISA - Q9UBU3

Core Product Specifications and Parameters

Biological Background: GHRL (Appetite-regulating Hormone) Function and Localization

• Encoded by the GHRL gene, the preproprotein is cleaved into ghrelin-27 and ghrelin-28, also known as growth hormone secretagogue, growth hormone-releasing peptide, and motilin-related peptide.
• Ghrelin is the endogenous ligand for the growth hormone secretagogue receptor type 1 (GHSR) and induces the release of growth hormone from the pituitary. Related references: PMID:10604470, PMID:35027551
• The hormone plays a central role in appetite stimulation, promoting adiposity and gastric acid secretion; it is involved in overall growth regulation.
• Octanoylation of ghrelin-28 is essential for GHSR activation, with octanoylated ghrelin being the most commonly found active form. Related references: PMID:11306336, PMID:35027551
• Secreted into the bloodstream, ghrelin exhibits highest expression in the stomach, with compensatory synthesis in other tissues following gastrectomy.
• Post-translational modifications include amidation and alternative splicing; ghrelin may also interact with GPR39 to influence gastric motility (by similarity).

Experimental Guidance and Technical Tips

• The immunogen covers the full-length human GHRL precursor (amino acids 1-117); the polyclonal antibody may recognize both the unprocessed precursor and mature ghrelin peptides.
• For Western blotting, the predicted molecular weight of the precursor is ~13 kDa, but post-translational processing can yield multiple bands; consider testing in stomach tissue lysates.
• In ELISA, the antibody can be explored as a capture or detection reagent; validate its performance in relevant sample matrices such as human serum or tissue extracts.
• Given the secretory nature of ghrelin, sample preparation should account for potential peptidase activity; use appropriate inhibitors if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive IVD raw-material solutions, including validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and rare-target sourcing. For researchers focused on appetite regulation and metabolic disorders, this anti-GHRL antibody provides a reliable tool to advance your diagnostic or research applications. As your one-stop partner, CamelBio supports every stage from concept to clinic with tailored technical and procurement services.