Anti-GHRH Polyclonal Antibody for WB, IF/ICC, ELISA - P01286

Core Product Specifications and Parameters

Biological Background: GHRH Function and Localization

• Somatoliberin, also known as growth hormone-releasing hormone (GHRH), growth hormone-releasing factor (GRF), somatocrinin, and somatorelin, is encoded by the GHRH gene and undergoes alternative splicing.
• GHRH is released by the hypothalamus and acts on the adenohypophysis to stimulate the secretion of growth hormone (GH), playing a central role in the regulation of somatic growth and metabolism.
• The protein is classified as a secreted hormone, consistent with its extracellular signaling function.
• Post-translational modifications include amidation and cleavage on a pair of basic residues, which are essential for its mature bioactive form.
• The presence of a signal peptide directs the nascent protein to the secretory pathway.
• Alternative splicing generates multiple isoforms, potentially diversifying the functionality of GHRH.
• The theoretical molecular weight of the unprocessed precursor is approximately 12.4 kDa (UniProt: 12447 Da), while the mature peptide is much smaller; the antibody recognizes a band around 12 kDa in relevant samples.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide covering amino acids 1-100 of human GHRH, which includes the signal peptide and part of the mature region; the antibody may detect both precursor and processed forms.
• For Western blot, consider analyzing tissue or cell lysates under reducing and non-reducing conditions to characterize the specific forms detected.
• In immunofluorescence/immunocytochemistry, nuclear or cytoplasmic localization is not expected; focus on secretory pathway structures and validate staining patterns with known controls.
• When performing ELISA, use appropriate peptide or recombinant protein standards to confirm assay specificity and linearity.
• Cross-reactivity with mouse and rat GHRH is indicated; however, it is advisable to verify sequence homology of the immunogen region across species before relying on this feature.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with streamlined access to a comprehensive portfolio of IVD raw materials, including validated antibody pairs and optimized polyclonal antibodies like this anti-GHRH reagent. For projects focused on growth hormone regulation and endocrine diagnostics, we offer bulk ancillary reagents and rapid sourcing of even rare-target raw materials. Let CamelBio be your reliable partner in moving from concept to clinical application.