Anti-GHRH Monoclonal Antibody for WB, ELISA - P01286

Core Product Specifications and Parameters

Biological Background: Somatoliberin Function and Localization

• Somatoliberin (also known as growth hormone-releasing hormone, GHRH, or growth hormone-releasing factor, GRF) is a hypothalamic peptide hormone that stimulates the secretion of growth hormone from the anterior pituitary gland.
• The protein is encoded by the GHRH gene, which produces a preproprotein that undergoes proteolytic processing to generate the mature, biologically active form.
• GHRH is a secreted signaling molecule, acting locally within the hypothalamic-pituitary axis to regulate somatotroph function.
• Post-translational modifications such as amidation and cleavage at basic residues are essential for the bioactivity and stability of the mature peptide.
• Alternative splicing of the GHRH gene yields multiple isoforms, potentially contributing to functional diversity.
• UniProt/Swiss-Prot annotation indicates that the protein has a molecular weight of approximately 12.4 kDa and is classified under reference proteome, with experimental confirmation by direct protein sequencing and proteomics identification.

Experimental Guidance and Technical Tips

• The immunogen consists of a recombinant protein covering amino acids 1-100 of human GHRH, which corresponds to the N-terminal region of the mature peptide. Consider verifying antibody specificity using recombinant GHRH or relevant control lysates.
• In Western blotting, a band around 12 kDa is expected for the unprocessed form; however, multiple bands may appear due to precursor processing or post-translational modifications. Use appropriate molecular weight markers and validate in the relevant sample system (e.g., hypothalamic or pituitary extracts).
• For ELISA applications, ensure that the antibody recognizes the native or properly folded antigen under assay conditions. Non-denaturing protocols may be beneficial.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement for diagnostic manufacturers and research laboratories, offering integrated access to validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. This anti-GHRH antibody supports investigations into growth hormone regulation and pituitary disorders, with CamelBio’s supply capability helping to accelerate development from concept to clinic.