Anti-GHD7 Polyclonal Antibody for WB, ELISA - E5RQA1

Core Product Specifications and Parameters

Biological Background: Transcription factor GHD7 Function and Localization

• Transcription factor GHD7 (Grain number, plant height and heading date 7) is a nuclear protein involved in flowering time regulation in rice (Oryza sativa).
• Functions as a floral repressor under long-day conditions, delaying flowering by downregulating the expression of EHD1 and HD3A.
• Belongs to a family of DNA-binding transcription factors; contains features consistent with a reference proteome and is annotated as a phosphoprotein.
• Localizes predominantly to the nucleus, consistent with its role in transcriptional regulation.
• Expressed in meristematic and developing tissues including the apical meristem, root meristem, developing leaves, leaf sheaths, epidermal layer of developing stems, and branch primordia of developing panicles.
• Plays a critical role in integrating environmental cues (photoperiod) to control the transition from vegetative to reproductive growth in rice.
• Key biological keywords: DNA-binding, flowering, nucleus, phosphoprotein, transcription, and transcription regulation.

Experimental Guidance and Technical Tips

• The recombinant protein immunogen suggests this antibody may recognize native GHD7 in rice lysates. For Western blot (WB), consider using rice tissue extracts known to express GHD7, such as developing leaves or meristematic tissues.
• Validate antibody specificity with appropriate controls, including negative tissue samples or knockout lines if available.
• ELISA may be suitable for detecting recombinant or native GHD7; optimize coating antigen and detection conditions.
• As a polyclonal antibody, assess lot-to-lot consistency and consider titrating the antibody for your specific assay.

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