Anti-Ghd 7.1 Rabbit Polyclonal Antibody for WB, ELISA - Q0D3B6

Core Product Specifications and Parameters

Biological Background: Ghd 7.1 Function and Localization

• Ghd 7.1 (PRR37) is a probable transcription factor that acts as a repressor of flowering under long day conditions. It controls flowering time by negatively regulating the expression of HD3A and, acting downstream of phytochrome B, represses EHD1, an activator of the flowering promoters HD3A and RFT1. Related references: PMID:23713079, PMID:25378698
• As a component of the circadian clock, Ghd 7.1 expression is controlled by circadian rhythm. It contributes to coordinated circadian waves with other PRR family members (PRR73, PRR95, PRR59, PPR1) that underlie the endogenous circadian clock (By similarity).
• Subcellular localization: Ghd 7.1 is localized to the nucleus, consistent with its role as a transcription regulator.
• Keyword associations: Biological rhythms, flowering, nucleus, phytochrome signaling pathway, transcription, transcription regulation, two-component regulatory system.
• Belongs to the pseudo-response regulator family in rice, with coordinated expression among PRR73, PRR37, PRR95, PRR59, and PPR1.

Experimental Guidance and Technical Tips

• The antibody is a rabbit polyclonal raised against an N-terminal recombinant immunogen (aa 1-100 of Ghd 7.1). For Western blotting, use appropriate Oryza sativa tissue lysates and verify the band at the predicted molecular weight of ~80 kDa; optimize antibody dilution to reduce potential background.
• For ELISA applications, coat the plate with the recombinant immunogen or target antigen and validate binding under your specific assay conditions. Cross-reactivity is reported only for Oryza sativa, so this antibody is suitable for rice-specific studies.
• Consider using the antibody in functional studies such as chromatin immunoprecipitation (ChIP) or immunofluorescence to confirm nuclear localization of Ghd 7.1, but these applications require additional validation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated one-stop sourcing partner for IVD raw materials, offering comprehensive support from concept to clinic. This anti-Ghd 7.1 polyclonal antibody supports research into rice flowering time regulation and circadian biology. With a wide portfolio of validated antibodies, bulk ancillary reagents, and tailored sourcing solutions, CamelBio ensures seamless procurement for diagnostic development and academic research.