Anti-γ-Tubulin Rabbit Polyclonal Antibody for WB, ELISA - P23258

Core Product Specifications and Parameters

Biological Background: γ-Tubulin Function and Localization

• The protein γ-Tubulin (Tubulin gamma-1 chain), encoded by the TUBG1 gene, is also known as Gamma-1-tubulin or GCP-1.
• Gamma-tubulin is a key component of the gamma-tubulin ring complex (γTuRC), which regulates minus-end nucleation of alpha-beta tubulin heterodimers into microtubule protofilaments, essential for centrosome duplication and spindle formation. Related references: PMID:38305685, PMID:38609661, PMID:39321809
• During late spermiogenesis, gamma-tubulin plays a role in anchoring the sperm head to the tail, contributing to proper sperm morphological development. Related references: PMID:38870534
• Subcellularly, γ-Tubulin localizes to the cytoplasm, cytoskeleton, microtubule organizing center, centrosome, and mitotic spindle.
• It is annotated as a phosphoprotein and GTP-binding protein, suggesting regulation by phosphorylation and potential nucleotide-dependent functions.
• Mutations in TUBG1 are associated with lissencephaly (smooth brain) and other cortical malformations, underscoring its importance in neurodevelopment.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 382–451 near the C-terminus of human γ-Tubulin; this region may be well-conserved across species, supporting the observed cross-reactivity with mouse and rat.
• For Western blotting, expect a band around 51 kDa in lysates from human, mouse, or rat cells. Consider including appropriate loading controls and validating detection in your specific sample system.
• ELISA applications may utilize the antibody as a capture or detection reagent; optimal titers and buffer conditions should be experimentally determined.
• Given its role at centrosomes and spindles, immunofluorescence or immunoprecipitation could be explorable, though not validated for this product.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For cytoskeleton and cell cycle research applications, including centrosome duplication and microtubule dynamics, this anti-γ-Tubulin antibody complements our portfolio of validated reagents and bulk ancillary materials. Explore our sourcing capabilities for rare targets and customized antibody production to accelerate your assay development.