Anti-γ-Tubulin Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P23258

Core Product Specifications and Parameters

Biological Background: γ-Tubulin Function and Localization

• γ-Tubulin (TUBG1) is the gamma-1 chain of tubulin, a major constituent of microtubules, distinct from the alpha- and beta-tubulin heterodimers that form the microtubule lattice.
• It is a key component of the gamma-tubulin ring complex (gTuRC) that mediates microtubule nucleation by regulating minus-end nucleation of alpha-beta tubulin heterodimers. Related references: PMID:38305685 PMID:38609661 PMID:39321809
• This nucleation process is critical for centrosome duplication and spindle formation during cell division. Related references: PMID:38305685 PMID:38609661 PMID:39321809
• γ-Tubulin also plays a role in sperm morphological development, specifically anchoring the sperm head to the tail during late spermiogenesis. Related references: PMID:38870534
• Subcellularly, γ-Tubulin localizes to the cytoplasm, centrosome, and spindle, consistent with its function at microtubule organizing centers.
• Post-translational modifications include phosphorylation, and the protein contains GTP-binding and nucleotide-binding domains, with mutations linked to lissencephaly.

Experimental Guidance and Technical Tips

• For Western blot (WB), a band at approximately 51 kDa is expected. Using appropriate loading controls and verifying antibody specificity with positive and negative lysates is recommended.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), adequate fixation, antigen retrieval, and permeabilization may be necessary; optimization for your specific sample type should be performed.
• The antibody's cross-reactivity with mouse and rat indicates high sequence conservation, suggesting potential reactivity with other species; validate in the relevant model system.
• For ELISA applications, consider pairing with a suitable detection antibody and optimizing coating conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a comprehensive provider of IVD raw materials, supporting diagnostic manufacturers and researchers from concept to clinic. For microtubule and centrosome-related research, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing of rare targets. Our integrated technical services and supply chain solutions are designed to streamline your assay development and production needs.