Anti-γ-Tubulin Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - P23258

Core Product Specifications and Parameters

Biological Background: γ-Tubulin Function and Localization

• Gamma-tubulin (TUBG1/GCP-1) is a key member of the tubulin superfamily and serves as the major gamma-tubulin isoform in human cells, forming the core of the gamma-tubulin ring complex (gTuRC).
• The gTuRC mediates microtubule nucleation by providing a template for alpha/beta-tubulin heterodimer assembly at the minus-ends, a critical process for cytoskeleton organization and cell division. Related references: PMID:38305685 PMID:38609661 PMID:39321809
• This structural protein localizes predominantly to the centrosome and spindle microtubule organizing centers (MTOCs) where it regulates centrosome duplication and mitotic spindle formation. Related references: PMID:38305685 PMID:38609661 PMID:39321809
• Gamma-tubulin also plays a specialized role in late spermiogenesis, facilitating the proper attachment of the sperm head to the tail by anchoring the microtubule-based manchette. Related references: PMID:38870534
• The protein is subject to post-translational modifications including phosphorylation, and contains GTP-binding domains, highlighted by keywords such as "Phosphoprotein", "GTP-binding", and "Nucleotide-binding".
• Clinically, mutations in TUBG1 are associated with lissencephaly and other neurodevelopmental disorders (Keyword: Lissencephaly).

Experimental Guidance and Technical Tips

• The recombinant protein immunogen was designed against full-length human γ-tubulin, suggesting potential recognition of multiple epitopes; consider validating specificity in knockout or knockdown systems.
• For western blotting, a band around 51 kDa is expected; conditions may need optimization for samples with abundant microtubule-associated proteins.
• In immunofluorescence/ICC, expect centrosomal and spindle staining in cycling cells; co-staining with α/β-tubulin or centrin may aid localization.
• The antibody's cross-reactivity with mouse and rat makes it suitable for rodent model studies of cell division, neurodevelopment, or spermatogenesis.
• For ELISA applications, assess linearity and sensitivity using recombinant γ-tubulin as a standard; matrix effects may vary between sample types.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of validated IVD antibodies, including this γ-Tubulin Rabbit Monoclonal Antibody, suitable for researchers and diagnostic developers focusing on cell cycle regulation, centrosome biology, or microtubule dynamics. Beyond this product, we supply bulk ancillary reagents and source rare-target raw materials to streamline your assay development pipeline from concept to clinic.