Anti-γ-Catenin Rabbit Polyclonal Antibody for WB, IP, ELISA - P14923

Core Product Specifications and Parameters

Biological Background: γ-Catenin Function and Localization

• Junction plakoglobin (γ-catenin), also known as catenin gamma, desmoplakin III, and desmoplakin-3, is encoded by the JUP gene and belongs to the armadillo repeat protein family, serving as a common component of both adherens junctions and desmosomes.
• As a junctional plaque protein, it occupies a strategic position at submembranous plaques to coordinate cytoskeletal organization, cell adhesion, and intercellular signaling.
• It can substitute for beta-catenin in E-cadherin/catenin adhesion complexes, linking cadherins to the actin cytoskeleton, and acts as a substrate for VE-PTP to promote VE-cadherin function in endothelial cells.
• The protein is localized to cell junctions (adherens junctions, desmosomes), cytoplasm, cytoskeleton, cell membrane, nucleus, and cell projections such as axons, reflecting its dynamic roles in cell architecture.
• Tissue-specific expression includes high levels in heart cardiomyocytes and skin keratinocytes, consistent with its importance in cardiac and epidermal integrity.
• Post-translational modifications include acetylation, phosphorylation, and glycosylation; mutations in JUP are linked to arrhythmogenic right ventricular cardiomyopathy (ARVC) and palmoplantar keratoderma.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 1-300, enabling recognition of the N-terminal region; consider using full-length γ-catenin as a positive control in your assays.
• For Western blotting, the predicted molecular weight is 82 kDa, though post-translational modifications may cause slight band shifts; validate detection in relevant tissue lysates (e.g., human or mouse heart, skin).
• In immunoprecipitation experiments, optimize antibody concentration and confirm co-precipitation of known binding partners such as cadherins or catenins.
• Antibody reactivity has been confirmed with human and mouse samples; cross-reactivity with other species should be tested individually.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research laboratories with ready access to a broad portfolio of IVD raw materials, including validated polyclonal and monoclonal antibodies, recombinant proteins, and ancillary reagents. This anti-γ-catenin antibody supports studies in cell junction biology and related pathologies, and we can assist with bulk orders, custom formulations, and sourcing of rare targets to accelerate your projects from concept to clinic. Reach out to learn how our integrated supply chain can become your reliable sourcing partner.