Anti-GGT5 Rabbit Polyclonal Antibody for WB and ELISA - P36269

Core Product Specifications and Parameters

Biological Background: GGT5 (Glutathione hydrolase 5 proenzyme) Function and Localization

• GGT5 encodes the glutathione hydrolase 5 proenzyme, also known as gamma-glutamyltransferase 5 (GGT5), GGT-rel, and leukotriene-C4 hydrolase; it belongs to the gamma-glutamyltransferase family and undergoes alternative splicing, yielding multiple isoforms.
• The enzyme cleaves the gamma-glutamyl bond of extracellular glutathione tripeptide and certain glutathione-S-conjugates, releasing L-glutamate and cysteinyl-glycine. This activity is essential for maintaining extracellular cysteine levels and providing cysteine for intracellular glutathione synthesis. Related references: PMID:1676842, PMID:21447318
• GGT5 specifically metabolizes leukotriene C4 (LTC4) to leukotriene D4 (LTD4) during acute inflammatory responses, and also hydrolyzes S-geranylgeranyl-glutathione (GGG), acting as a negative regulator of GGG bioactivity. Together with ABCC1, it establishes GGG gradients that guide P2RY8-positive lymphocyte positioning within lymphoid follicles (By similarity).
• The transpeptidation reaction, which transfers the gamma-glutamyl moiety to an acceptor molecule, requires high dipeptide acceptor concentrations and is considered nonphysiological. Related references: PMID:29667297
• As a membrane-bound glycoprotein, GGT5 features a signal-anchor and a transmembrane helix. It is synthesized as an inactive proenzyme (zymogen) that likely requires proteolytic cleavage for activation.
• At the protein level, GGT5 is expressed in follicular dendritic cells within germinal centers of lymphoid follicles, underscoring its role in immune function.
• Functional keywords include acyltransferase, alternative splicing, glutathione biosynthesis, glycoprotein, hydrolase, leukotriene biosynthesis, membrane, protease, transferase, and zymogen, highlighting its multifunctional nature.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 388-587 of human GGT5; this region is predicted to be conserved in mouse, consistent with the stated cross-reactivity. Users should validate detection of endogenous protein in their specific sample matrix.
• For western blot, anticipate a band near 62 kDa, but be aware that glycosylation and proenzyme processing may produce additional bands or slight shifts. Reducing/non-reducing conditions may influence migration.
• In ELISA, perform titration experiments to determine optimal antibody and antigen concentrations. Include appropriate controls to assess specificity.
• This polyclonal antibody may be suitable for detecting both proenzyme and cleaved forms; further characterization in the intended application is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive partner for IVD raw materials, offering everything from validated antibody pairs and recombinant proteins to bulk ancillary reagents and custom sourcing solutions. This anti-GGT5 polyclonal antibody supports researchers studying glutathione metabolism, leukotriene signaling, and immune cell positioning. Whether you require optimized monoclonals, polyclonals, or hard-to-find target reagents, CamelBio streamlines your procurement from concept to clinic.