Anti-GGPS1 Polyclonal Antibody for WB, ELISA - O95749

Core Product Specifications and Parameters

Biological Background: GGPS1 Function and Localization

• GGPS1 encodes geranylgeranyl pyrophosphate synthase, also known as (2E,6E)-farnesyl diphosphate synthase, dimethylallyltranstransferase, farnesyl diphosphate synthase, farnesyltranstransferase, geranylgeranyl diphosphate synthase, or geranyltranstransferase, and is also referred to by aliases GGPPS, MDHLO, and GGPPS1.
• This enzyme catalyzes the trans-addition of three molecules of isopentenyl pyrophosphate (IPP) onto dimethylallyl pyrophosphate (DMAPP) to form geranylgeranyl pyrophosphate, a key precursor in the biosynthesis of carotenoids and geranylated proteins.
• Geranylgeranyl pyrophosphate synthase belongs to the FPP/GGPP synthase family of prenyltransferases.
• Subcellular localization studies reveal that GGPS1 localizes to the cytoplasm, with enrichment in the perinuclear region and association with myofibrils, specifically the sarcomere Z line.
• GGPS1 is abundantly expressed in testis, with lower levels observed in other tissues; it is also detected in dermal fibroblasts and skeletal muscle. Related references: PMID:10026212 PMID:9741684 PMID:32403198
• The protein is subject to acetylation and is characterized by keywords such as metal-binding and magnesium cofactor binding.
• Alternative splicing of GGPS1 yields multiple transcript variants, and mutations in this gene are associated with congenital muscular dystrophy and deafness.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody corresponds to amino acids 164-300 of human GGPS1 (based on NP_001032354.1), covering a central region of the protein. This region may allow recognition of endogenous GGPS1 across species, but cross-reactivity should be validated in the relevant experimental system.
• For Western blot (WB), a band around 35 kDa is expected based on the reported protein weight. However, post-translational modifications or alternative splicing may result in additional bands; validation with proper controls (e.g., positive lysates from testis or skeletal muscle) is recommended.
• When using this antibody in ELISA, consider optimizing coating conditions and detection parameters for each specific assay format, as indirect ELISA performance can vary with different antigen presentation.
• For tissue-specific studies, note that GGPS1 is highly expressed in testis and also found in skeletal muscle and dermal fibroblasts; expression levels should be confirmed in your cell or tissue model.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research groups by offering reliable access to high-quality IVD raw materials. For targets like GGPS1, involved in isoprenoid biosynthesis and linked to muscular dystrophy and deafness, we can supply validated antibodies (including polyclonal and monoclonal formats), antibody pairs, and bulk ancillary reagents to streamline your assay development. Reach out to explore how our one-stop sourcing services can accelerate your project, from early biomarker discovery to final kit integration.