Anti-GGCX Rabbit Polyclonal Antibody for WB and ELISA - P38435

Core Product Specifications and Parameters

Biological Background: GGCX Function and Localization

• GGCX is a vitamin K-dependent gamma-carboxylase that catalyzes the post-translational carboxylation of glutamate residues to gamma-carboxyglutamate (Gla), enabling calcium binding and protein activation. Related references: PMID:17073445, PMID:39880952, PMID:39880037

• It carboxylates blood coagulation factors (II, VII, IX, X) and anticoagulant protein C, directly regulating hemostasis and thrombosis. Related references: PMID:17073445, PMID:39880037

• GGCX modifies osteocalcin (BGLAP) and matrix Gla protein (MGP), playing essential roles in bone metabolism and vascular calcification. Related references: PMID:17073445, PMID:39880952, PMID:39880037

• The enzyme localizes to the endoplasmic reticulum membrane, where it processes secretory and transmembrane substrates.

• Post-translational modifications include acetylation, glycosylation, and disulfide bond formation, as identified by proteomics and structural studies.

• Mutations in GGCX cause the bleeding disorder VKCFD1 and are associated with abnormal calcification phenotypes.

Experimental Guidance and Technical Tips

• The immunogen (aa 479–758, C‑terminal region) may detect full‑length GGCX (~88 kDa) and naturally occurring isoforms; validate band patterns in relevant sample types.
• For Western blot, use human or mouse cell lysates known to express coagulation factors (e.g., liver) as positive controls; optimize loading amounts and antibody dilutions.
• In ELISA applications, consider coating with recombinant GGCX protein or peptide immunogen and confirm specificity through competition assays.
• Cross‑reactivity with mouse GGCX is confirmed; additional species should be verified individually due to sequence variations.

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CamelBio supports diagnostic manufacturers and research institutes with comprehensive IVD raw‑material supply, including validated antibodies like this GGCX polyclonal antibody for coagulation and bone metabolism studies. We offer antibody pairs, bulk ancillary reagents, and sourcing for rare targets, streamlining your progression from concept to clinic. Partner with us to secure reliable, high‑quality materials for your assay development needs.