Anti-GGCT Polyclonal Antibody for WB, ELISA - O75223

Core Product Specifications and Parameters

Biological Background: Gamma-glutamylcyclotransferase Function and Localization

• Gamma-glutamylcyclotransferase (GGCT), also known as cytochrome c-releasing factor 21 (CRF21), is encoded by the GGCT gene (C7orf24) in Homo sapiens.
• GGCT catalyzes the formation of 5-oxoproline from gamma-glutamyl dipeptides, playing a significant role in glutathione homeostasis.
  Related references:
  PMID:18515354
• It induces the release of cytochrome c from mitochondria, resulting in apoptosis.
  Related references:
  PMID:16765912
• The protein is annotated as a lyase (EC 4.3.2.7) and phosphoprotein, and its three-dimensional structure has been determined.
• Alternative splicing yields multiple isoforms.
• No specific subcellular localization or tissue specificity data is available in the current UniProt annotation.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein covering the full-length human GGCT (aa 1-188), suggesting this polyclonal antibody can recognize both native and denatured forms; it is suitable for WB under standard reducing conditions and ELISA.
• For Western blotting, a single band around 21 kDa is expected; consider using lysates from GGCT-expressing cell lines (e.g., HeLa, HEK293) or tissues as positive controls.
• In ELISA, the antibody can be employed as a capture or detection reagent; validate pairing with antigen standards for quantitative assays.
• Cross-reactivity is currently documented for human only; if applying to other species, perform independent validation.
• Due to the polyclonal nature, lot-to-lot consistency may vary; we recommend establishing internal reference standards for critical applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement for diagnostic manufacturers, offering validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. This anti-GGCT antibody supports research into glutathione metabolism and apoptosis, serving as a foundation for assay development. Our team also specializes in sourcing rare-target antibodies and scaling up reagent supplies to meet your project timelines.