Anti-GGA2 Polyclonal Antibody for WB, IF/ICC, ELISA - Q9UJY4

Core Product Specifications and Parameters

Biological Background: GGA2 Function and Localization

• GGA2 (ADP-ribosylation factor-binding protein GGA2) is a member of the Golgi-localized, gamma ear-containing, ARF-binding protein family, functioning as an adaptor in vesicular transport between the trans-Golgi network (TGN) and endosomes.
• Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (DXXLL) motif. Related references: PMID:10747088
• Plays a role in the cell surface export of the GPCR receptor ADRA2B. Related references: PMID:27901063
• Regulates the retrograde transport of the phosphorylated form of BACE1 from endosomes to the trans-Golgi network. Related references: PMID:15615712
• Localizes predominantly to the trans-Golgi network membrane and early endosome membrane, consistent with its sorting and trafficking roles.
• Ubiquitously expressed across tissues, indicating broad cellular functions.
• Classified as a phosphoprotein and is involved in protein transport; also identified in proteomics studies and subject to ubiquitin conjugation.

Experimental Guidance and Technical Tips

• For Western blot analysis, a band near 67 kDa is expected in human and mouse cell lysates; consider optimizing lysis and electrophoresis conditions for clear detection.
• In IF/ICC experiments, appropriate fixation and permeabilization protocols may affect staining patterns; validate with known subcellular markers and controls.
• Owing to its polyclonal nature, the antibody may exhibit robust cross-reactivity; nevertheless, confirm species specificity and signal linearity in the intended assay system.
• For ELISA, consider using recombinant GGA2 protein as a standard and titrate antibody concentration to establish a sensitive detection range.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to validated IVD raw materials. This GGA2 polyclonal antibody is suited for investigations into intracellular trafficking and Golgi-endosome biology, and CamelBio can further support your projects with bulk ancillary reagents, optimized antibody pairs, and rare-target sourcing. Let us help streamline your workflow from concept to clinic.