Anti-GFRA2 Rabbit Polyclonal Antibody for WB and ELISA - O00451

Core Product Specifications and Parameters

Biological Background: GFRA2 Function and Localization

• GFRA2 (GDNF family receptor alpha-2) serves as the primary receptor for neurturin (NRTN), a neurotrophic factor critical for the survival of sympathetic neurons.
  Related references: PMID:10829012, PMID:29414779, PMID:31535977

• NRTN binding to GFRA2 induces autophosphorylation and activation of the RET tyrosine kinase receptor, initiating downstream signaling cascades.
  Related references: PMID:10829012, PMID:29414779, PMID:31535977

• GFRA2 can also mediate GDNF signaling through RET, expanding its functional repertoire in neurotrophic signaling.
  Related references: PMID:9182803

• Participates in NRTN-induced phosphorylation of STAT3 at Ser-727, linking to transcriptional regulation.

• Anchored to the cell membrane via a GPI-anchor, consistent with its role as a plasma membrane receptor.

• Expressed in the brain and placenta, reflecting its role in neuronal and possibly placental functions.

• Post-translational modifications include N-linked glycosylation and disulfide bond formation, which may affect protein stability and function.

• Also known as GDNFR-beta, NRTNR-alpha, RETL2, and TRNR2, indicating multiple recognition sites and functional domains.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 360-464 of human GFRA2, covering a C-terminal region; this design may facilitate detection of both full-length protein and potential isoforms.
• For Western blot, the predicted molecular weight is 52 kDa, but glycosylation may cause a shift; validate molecular weight markers and lysate conditions.
• This polyclonal antibody is validated for ELISA; consider optimizing coating antigen concentration and detection system for best signal-to-noise ratio.
• Cross-reactivity with mouse and rat has been reported; always include species-matched positive and negative controls in your experiments.
• As a membrane protein, effective extraction may require the use of detergent-containing lysis buffers suitable for GPI-anchored proteins.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive portfolio of high-quality IVD raw materials, including this GFRA2 Rabbit pAb for researchers studying neurotrophic signaling and sympathetic neuron biology. As your one-stop sourcing partner, we offer validated antibody pairs, bulk reagents, and customized raw-material solutions to support your diagnostic development from concept to clinic. Contact us to explore our end-to-end supply capabilities and technical consultation services.