Anti-GFRA1 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P56159

Core Product Specifications and Parameters

Biological Background: GFRA1 Function and Localization

• GFRA1 (GDNF family receptor alpha-1) is a GPI-anchored coreceptor for the neurotrophic factor GDNF, essential for the development and maintenance of dopaminergic neurons. Related references: PMID:10829012 PMID:31535977
• Binding of GDNF to GFRA1 leads to autophosphorylation and activation of the RET receptor tyrosine kinase, propagating downstream signaling pathways. Related references: PMID:31535977
• The protein localizes to the cell membrane, Golgi apparatus, trans-Golgi network, endosomes, and multivesicular bodies, indicative of dynamic trafficking.
• GFRA1 is a glycoprotein with multiple disulfide bonds and a C-terminal GPI-anchor for membrane attachment. Alternative splicing generates several isoforms.
• Mutations in GFRA1 have been associated with disease variants.
• The calculated molecular weight of the mature protein is approximately 51 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 345–420 in the C-terminal region of human GFRA1, suggesting the antibody may recognize the extracellular domain near the GPI anchor site.
• For Western blotting, the expected band is around 51 kDa; however, glycosylation may shift the apparent molecular weight. Validate band specificity with appropriate positive and negative controls.
• In IHC-P, consider antigen retrieval methods and use brain or neuronal tissue sections as positive controls, where GFRA1 is highly expressed.
• For IF/ICC, permeabilization is recommended to detect intracellular pools; co-staining with organelle markers can confirm localization.
• Always titrate the antibody and optimize detection conditions for your specific sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines your IVD development with one-stop access to raw materials, from validated antibody pairs and optimized monoclonal/polyclonal antibodies to bulk ancillary reagents. Our GFRA1 polyclonal antibody supports neurobiology and cancer research, accelerating assay development for diagnostic manufacturers. With end-to-end sourcing from concept to clinic, CamelBio is your trusted partner for rare target raw-material procurement.