Anti-GFAP Rabbit Polyclonal Antibody for WB, IF/ICC, IF-P, IHC-P, and ELISA - P14136

Core Product Specifications and Parameters

Biological Background: GFAP Function and Localization

• GFAP is a class-III intermediate filament protein and a well-established astrocyte-specific marker during central nervous system development.
• It localizes predominantly to the cytoplasm, forming the structural framework of intermediate filaments in astrocytes.
• GFAP is expressed in cells that lack fibronectin, reflecting its tissue-specific distribution in glial cells.
• The protein undergoes various post-translational modifications including phosphorylation, citrullination, and methylation, which may regulate filament dynamics and function.
• Alternative splicing generates multiple isoforms, contributing to functional diversity.
• Mutations in GFAP are associated with leukodystrophy, highlighting its role in white matter integrity.
• As a reference proteome component, GFAP is widely used in proteomics and diagnostic applications.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-75) of human GFAP, which is highly conserved across human, mouse, and rat, supporting the observed cross-reactivity.
• For Western blot (WB), a specific band around 50 kDa should be detected, aligning with the predicted molecular weight of approximately 49.9 kDa.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC, IF-P), proper fixation and antigen retrieval methods should be optimized for each sample type.
• ELISA applications can be used to quantify GFAP levels, but assay conditions need to be empirically determined.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive partner for IVD raw materials, offering a full range of validated antibodies and bulk reagents for neuroscience research. Our GFAP rabbit polyclonal antibody is ideal for astrocyte marker studies, and we also provide optimized antibody pairs, monoclonal alternatives, and ancillary reagents to streamline your diagnostic development. Leverage our one-stop sourcing to accelerate your projects from concept to clinic.