Anti-GFAP Polyclonal Antibody - P14136

Core Product Specifications and Parameters

Biological Background: GFAP Function and Localization

• Glial fibrillary acidic protein (GFAP) is a class-III intermediate filament protein that serves as an established cell-type-specific marker for astrocytes during central nervous system development, distinguishing them from other glial cells.
• The protein is predominantly localized in the cytoplasm, contributing to the cytoskeletal architecture of astrocytes.
• GFAP expression has been noted in cells lacking fibronectin, indicating a specialized tissue distribution pattern.
• As a member of the intermediate filament family, GFAP contains a central coiled-coil rod domain and is subject to multiple post-translational modifications including phosphorylation, citrullination, and methylation.
• Alternative splicing generates multiple isoforms of GFAP, which may have distinct functional roles in different cell contexts.
• Clinically, variants in GFAP are linked to leukodystrophy (based on UniProt keyword annotations), underscoring its importance in neurological disorders.

Experimental Guidance and Technical Tips

• This polyclonal antibody was raised against a synthetic peptide corresponding to amino acids 350-432 of human GFAP, and is expected to recognize the target in denatured samples (e.g., WB) due to the linear epitope nature.
• For WB, a band around 50 kDa is anticipated; consider using positive controls such as brain tissue lysates or astrocyte cell lines, and validate specificity in relevant knockdown or knockout sample systems if available.
• In IF/ICC and IHC-P, verify that the epitope is accessible in fixed/permeabilized samples, as cross-linking may mask the peptide region; standard antigen retrieval methods may improve detection.
• Cross-reactivity with mouse and rat has been reported, but it is advisable to confirm reactivity in the specific species and tissue context of interest before large-scale experiments.
• The antibody is also applicable in ELISA; consider using it as a capture or detection reagent after in-house optimization of assay conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with streamlined access to high-quality IVD raw materials, including validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. For neuroscience-related projects focusing on astrocyte biology and neurodegenerative markers like GFAP, we are well-positioned to support your sourcing needs—from initial development to clinical application. Whether you require rare-target reagents or scalable production of existing immunoreagents, CamelBio bridges the gap between concept and clinic with reliable supply solutions.