Anti-GFAP Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - P14136

Core Product Specifications and Parameters

Biological Background: GFAP Function and Localization

• GFAP (Glial fibrillary acidic protein) is a class-III intermediate filament protein and a classical marker for astrocytes in the central nervous system.
• During CNS development, GFAP expression distinguishes astrocytes from other glial cells.
• Belongs to the intermediate filament family; other class-III members include vimentin, desmin, and peripherin.
• Localizes predominantly in the cytoplasm, contributing to the cytoskeletal network of astrocytes.
• Post-translational modifications include citrullination, methylation, and phosphorylation, which may regulate filament assembly and dynamics.
• Tissue expression is observed in cells lacking fibronectin.
• Associated with disease variants in leukodystrophy, notably Alexander disease.
• Alternative splicing generates multiple isoforms with potential functional diversity.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 60–383 of human GFAP; consider verifying recognition of all isoforms in your experimental system.
• For Western blot, a band at ~50 kDa is expected; validate using lysates from astrocyte-rich tissues or cell lines.
• For IHC-P and IF-P, brain sections from human, mouse, or rat provide suitable positive controls; optimize antigen retrieval and fixation conditions as needed.
• In ELISA, the recombinant immunogen can serve as a coating antigen; assess cross-reactivity with other intermediate filament proteins if multiplexing.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, bulk monoclonal antibodies, and ancillary reagents. The GFAP monoclonal antibody targets a key astrocyte marker, supporting neurology-related assay development. Partner with CamelBio for one-stop access to rare target sourcing and tailored supply solutions.