Anti-GFAP Monoclonal Antibody for WB, IF-F, IF-P, mIHC, ELISA - P14136

Core Product Specifications and Parameters

Biological Background: GFAP Function and Localization

• GFAP is a class-III intermediate filament protein and a cell-specific marker for astrocytes in the central nervous system, distinguishing them from other glial cells.
• It is encoded by the GFAP gene in humans (GeneID: 2670).
• Subcellular localization: primarily cytoplasmic.
• Tissue specificity: expressed in cells lacking fibronectin, notably in astrocytes.
• Post-translational modifications include citrullination, methylation, and phosphorylation (from UniProt keywords).
• Associated with disease: mutations linked to leukodystrophy (Alexander disease).
• The protein has a molecular weight of approximately 50 kDa (calculated 49880 Da).

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide; consider using a blocking peptide for specificity control in immunoassays.
• For Western blot (WB), the expected band is around 50 kDa. Validate with positive control lysates from brain or astrocyte cell lines.
• For immunofluorescence (IF-F and IF-P), ensure appropriate fixation and permeabilization for cytoplasmic targets.
• For multiplex IHC (mIHC), check compatibility with other primary antibodies, especially regarding cross-reactivity and species.
• For ELISA, the antibody may be used for capture or detection; optimize coating and detection conditions.

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CamelBio offers a comprehensive range of IVD raw materials, including validated monoclonal antibodies like this anti-GFAP rabbit mAb, for neuroscience and diagnostic applications. From bulk reagents to rare target sourcing, our supply chain supports your assay development needs. Partner with us to streamline your procurement from concept to clinic.