Anti-GFAP Monoclonal Antibody (Conjugate) for Immunofluorescence - P14136

Core Product Specifications and Parameters

Biological Background: GFAP Function and Localization

• GFAP is a class-III intermediate filament protein and a well-established cell-specific marker for astrocytes, distinguishing them from other glial cells during central nervous system development.
• It localizes predominantly in the cytoplasm, where it contributes to the cytoskeletal network of intermediate filaments.
• Alternative splicing of the GFAP gene produces multiple isoforms, which may have distinct expression patterns and functional roles.
• GFAP is expressed in cells lacking fibronectin, indicating a specific cellular context related to astrocyte identity.
• Post-translational modifications include citrullination, phosphorylation, and methylation, which can regulate filament assembly and dynamics.
• Mutations in GFAP are associated with disease variants, including Alexander disease (a type of leukodystrophy), underscoring its clinical relevance.

Experimental Guidance and Technical Tips

• For immunofluorescence applications, optimize fixation and permeabilization conditions to preserve antigenicity and achieve clear astrocyte-specific staining.
• Confirm cross-reactivity in mouse and rat tissues by validating in relevant brain sections or primary astrocyte cultures to ensure species-specific performance.
• As a rabbit monoclonal antibody, expect consistent batch-to-batch performance; however, always titrate the antibody in your specific assay system to determine the optimal working dilution.
• The immunogen corresponds to the N-terminal region (aa 1–100) of human GFAP; note that some splice variants or disease-associated mutations in this region may affect antibody recognition, so validate in your target sample if relevant.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio enables diagnostic manufacturers and research labs to efficiently source validated IVD raw materials, including this anti-GFAP monoclonal antibody for neurological biomarker studies and astrocyte-targeted assay development. We offer bulk supplies of optimized antibodies, ancillary reagents, and streamlined procurement of rare-target raw materials to accelerate your project from concept to clinic. Partner with CamelBio to simplify your supply chain and secure high-quality immunological reagents tailored to your needs.