Anti-GF14-C Rabbit Polyclonal Antibody for WB and ELISA - Q6ZKC0

Core Product Specifications and Parameters

Biological Background: GF14-C Function and Localization

• 14-3-3-like protein GF14-C, also known as G-box factor 14-3-3 homolog C, is a rice protein encoded by the GF14C gene (Gene ID: 4345634).
• It functions as part of a DNA-binding complex that specifically interacts with the G-box, a conserved cis-acting DNA regulatory element present in various plant gene promoters, thereby implicating it in transcriptional regulation.
• The protein localizes to both the cytoplasm and nucleus, consistent with its role in signal transduction and gene expression.
• Tissue-specific expression analysis reveals GF14-C in seedlings, internodes, and panicles, suggesting involvement in plant growth and reproductive development.
• As a member of the 14-3-3 protein family, it likely participates in protein-protein interactions and phosphoserine/phosphothreonine binding, modulating key cellular processes.
• Keywords include 3D-structure and reference proteome, indicating its structural characterization and foundational importance in rice proteomics.

Experimental Guidance and Technical Tips

• For Western blot, the recombinant immunogen suggests the antibody may recognize the denatured form; however, validate the expected band size (~71kDa) in your rice protein extracts, noting that endogenous GF14-C has a calculated molecular weight of ~28.8kDa, so the 71kDa observation may reflect post-translational modifications or oligomerization.
• This polyclonal antibody is suitable for ELISA; consider optimizing coating antigen and antibody dilutions using purified recombinant GF14-C or rice tissue lysates.
• Since cross-reactivity is reported only for Oryza sativa, validate any potential reactivity with closely related plant species if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories a single-source solution for IVD raw materials, including the GF14-C Rabbit pAb and complementary reagents for plant biology applications. Our supply scope includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets like rice 14-3-3 proteins. Partner with CamelBio to streamline your workflow from concept to clinic.