Anti-GEN1 Rabbit Polyclonal Antibody for WB and ELISA - Q17RS7

Core Product Specifications and Parameters

Biological Background: GEN1 Function and Localization

• GEN1 (Flap endonuclease GEN homolog 1) is a nuclear endonuclease that resolves Holliday junctions (HJs), four-way DNA intermediates formed during homologous recombination and DNA repair.
  Related references: PMID:19020614 PMID:26682650
• It cleaves HJs by a coordinated nick and counter-nick mechanism, requiring dimerization on the DNA junction for the first rate-limiting incision, followed by rapid second-strand cleavage.
  Related references: PMID:26578604
• GEN1 also exhibits endonuclease activity on 5'-flap and replication fork DNA substrates, contributing to the processing of branched DNA structures.
  Related references: PMID:26578604
• The enzyme displays a weak sequence preference for incising between two guanine residues located in a T-rich DNA region.
  Related references: PMID:28049850
• Localized to the nucleus, GEN1 plays a critical role in maintaining genomic integrity through DNA damage repair and proper chromosome segregation.
• Post-translational modifications include phosphorylation, which may regulate its activity or interactions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (amino acids 669-908) of human GEN1, potentially enabling detection of both full-length and truncated forms.
• For Western blot analysis, consider using whole-cell or nuclear extracts from human or mouse cell lines, given the nuclear localization of GEN1.
• Validate antibody specificity using appropriate controls, such as GEN1 knockout or knockdown cell lysates.
• In ELISA applications, test both direct and sandwich formats using recombinant GEN1 protein to optimize assay conditions.
• As a polyclonal antibody, be aware of potential lot-to-lot variability and perform routine quality checks.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with streamlined access to high-quality IVD antibodies and related raw materials. This anti-GEN1 polyclonal antibody supports research into DNA repair and genomic integrity, and CamelBio can further supply validated antibody pairs, optimized monoclonal alternatives, and ancillary reagents to accelerate your assay development. Our team also specializes in sourcing rare-target raw materials to meet specialized project requirements.