Anti-GEMIN8 Rabbit Polyclonal Antibody for WB, ELISA - Q9NWZ8

Core Product Specifications and Parameters

Biological Background: Gem-associated protein 8 Function and Localization

• Gem-associated protein 8 (GEMIN8), also known as protein FAM51A1, is a component of the survival motor neuron (SMN) complex.
• The SMN complex catalyzes the assembly of small nuclear ribonucleoproteins (snRNPs), which are essential building blocks of the spliceosome for pre-mRNA splicing.
• GEMIN8 participates in the formation of the Sm core of spliceosomal snRNPs by facilitating the transfer of Sm proteins (SNRPD1, SNRPD2, SNRPE, SNRPF, SNRPG) from the CLNS1A chaperone to the SMN complex.
• It localizes primarily to the nucleus (specifically gems) and cytoplasm, consistent with its role in snRNP biogenesis and trafficking.
• The protein is identified by keywords including mRNA processing, mRNA splicing, and phosphoprotein, and it contains a coiled-coil domain.
• The calculated molecular weight is approximately 28.6 kDa, while the observed weight is around 29 kDa; the immunogen corresponds to the full-length human GEMIN8 (amino acids 1-242).

Experimental Guidance and Technical Tips

• For Western blotting, consider using standard loading controls and validate the antibody in human cell lines or tissues that express GEMIN8 to confirm specificity and optimal dilution.
• The antibody is suitable for ELISA; optimize antigen coating concentration and blocking conditions experimentally for best signal-to-noise ratio.
• As reactivity is confirmed only with human, cross-reactivity with other species should be tested independently if needed.
• Given that GEMIN8 is a phosphoprotein, consider the use of phosphatase inhibitors in lysis buffers if phosphorylation states are of interest.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for diagnostic manufacturers and research laboratories, offering a wide range of IVD raw materials including validated antibodies against critical targets like GEMIN8 involved in mRNA splicing and snRNP assembly. Our supply capabilities encompass validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and support for rare target material sourcing, helping to streamline your development from concept to clinic.