Anti-GEMIN5 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q8TEQ6

Core Product Specifications and Parameters

Biological Background: GEMIN5 Function and Localization

• Gem-associated protein 5 is a component of the survival motor neuron (SMN) complex, which catalyzes the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome, playing a crucial role in pre-mRNA splicing. Related references: PMID:16857593, PMID:18984161, PMID:20513430
• GEMIN5 specifically recognizes and delivers small nuclear RNAs (snRNAs) to the SMN complex by binding to the 7-methylguanosine cap structure of RNA molecules. Related references: PMID:11714716, PMID:16314521, PMID:16857593, PMID:19750007, PMID:27834343, PMID:27881600, PMID:27881601
• Within the SMN complex, GEMIN5 facilitates the handover of snRNA to core Sm proteins, promoting the assembly of the spliceosomal core snRNP.
• GEMIN5 binds the 3'-UTR of SMN1 mRNA, regulating its translation without affecting mRNA stability. Related references: PMID:25911097
• May also play a role in the regulation of protein synthesis through its interaction with ribosomes. Related references: PMID:27507887
• Subcellular localization includes the nucleoplasm, nuclear gems, and cytoplasm, reflecting its dynamic roles in snRNP biogenesis and possibly translation.
• Belongs to the WD repeat-containing protein family, containing multiple WD repeats that mediate protein-protein and protein-RNA interactions.
• Post-translational modifications include phosphorylation and isopeptide bond formation, and it is implicated in disease (disease-associated variant).

Experimental Guidance and Technical Tips

• Since this rabbit polyclonal antibody was generated against a recombinant fragment (aa 750-950), it is expected to recognize multiple epitopes within this region, potentially providing robust detection across isoforms.
• For Western blot, given the large protein size (~169 kDa), consider using a low-percentage SDS-PAGE gel (e.g., 6% or gradient) and optimize transfer conditions to ensure efficient blotting of high-molecular-weight proteins.
• For IHC-P, validate in your specific tissue by testing various antigen retrieval methods and antibody dilutions, as the large size may influence accessibility.
• Cross-reactivity with mouse and rat suggests the target epitopes are conserved; however, verify band specificity in these species by using positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your comprehensive one-stop bridge for IVD raw materials, offering validated antibodies such as this GEMIN5 polyclonal antibody for research into snRNP biogenesis and splicing mechanisms. We provide optimized monoclonal and polyclonal antibodies, validated antibody pairs, bulk ancillary reagents, and customized sourcing for rare targets, supporting your diagnostic development from concept to clinic.