Anti-Gemin 2 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - O14893

Core Product Specifications and Parameters

Biological Background: Gemin 2 Function and Localization

• GEMIN2 is a central component of the essential SMN complex that catalyzes the assembly of spliceosomal snRNPs, the building blocks of the spliceosome required for pre-mRNA splicing. Related references: PMID:18984161 PMID:9323129

• Within the SMN complex, GEMIN2 constrains the conformation of the 5Sm protein subcomplex, thereby promoting selective binding to snRNAs containing the snRNP code and preventing misassembly. Related references: PMID:16314521 PMID:21816274 PMID:31799625

• GEMIN2 localizes to both the nucleus, where it is enriched in gem (Gemini of coiled bodies) structures, and the cytoplasm, reflecting its dynamic role in snRNP biogenesis and transport.

• GEMIN2 is subject to post-translational phosphorylation and the gene undergoes alternative initiation and alternative splicing, generating multiple protein isoforms that may fine-tune its activity.

• The GEMIN2 gene (also known as SIP1) is widely expressed, and its protein product is identified as part of the reference proteome, underscoring its fundamental importance in RNA processing.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa1–100); consider this epitope location when designing truncations or studying protein interactions.
• For Western blotting under denaturing and reducing conditions, expect a band near 32 kDa; phosphatase treatment may reveal phosphorylation-dependent mobility shifts.
• In immunohistochemistry (paraffin-embedded sections), standard antigen retrieval is recommended; validate staining patterns in relevant nuclear/subnuclear compartments.
• Direct or sandwich ELISA can be developed using the recombinant protein or peptide; confirm linearity with target analyte in your sample matrix.
• Because the antibody cross-reacts with mouse and rat Gemin 2, it is suitable for comparative studies across these species; verify sequence identity if extending to other organisms.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted one-stop partner for IVD raw materials, connecting diagnostic developers and laboratories with high-quality reagents like this anti-Gemin 2 monoclonal antibody to accelerate spliceosome and RNA-processing research. Our portfolio includes validated antibody pairs, optimized monoclonals/polyclonals, bulk ancillary reagents, and custom sourcing for rare targets, supporting your workflow from concept to clinic.