Anti-Gelsolin Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P06396

Core Product Specifications and Parameters

Biological Background: Gelsolin Function and Localization

• Encoded by the GSN gene, Gelsolin (also known as Actin-depolymerizing factor/ADF, Brevin, AGEL) is a calcium-regulated actin-binding protein.
• It modulates actin dynamics by capping the barbed ends of actin filaments, nucleating actin polymerization, and severing pre-formed filaments. Related references: PMID:19666512
• Plays a role in ciliogenesis. Related references: PMID:20393563
• Subcellularly localized to the cytoplasm and cytoskeleton, and can be secreted.
• Expressed in phagocytic cells, platelets, fibroblasts, nonmuscle cells, and smooth and skeletal muscle cells.
• Key post-translational modifications include phosphorylation and acetylation; contains disulfide bonds and metal-binding sites.
• Associated with diseases such as amyloidosis and corneal dystrophy (based on UniProt keywords).

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein spanning amino acids 28–782 of human Gelsolin, covering the core actin-binding domains; this broad coverage may yield reactivity across isoforms.
• For Western blot (WB), a band corresponding to ~86 kDa is expected; validate with size markers and appropriate positive controls.
• The antibody is suitable for WB, IHC-P, and ELISA; cross-reactivity with mouse and rat allows multi-species studies.
• Due to Gelsolin’s calcium-dependent conformation, buffer conditions containing calcium may be critical for maintaining native epitope structure in certain assays.
• For IHC-P, optimize antigen retrieval and include relevant tissue controls such as phagocytic cells, smooth muscle, or fibroblasts.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with comprehensive IVD raw-material sourcing, from validated antibody pairs to bulk ancillary reagents. Our expertise in cytoskeleton biology and rare-target antibodies ensures reliable access to specialized tools like this anti-Gelsolin polyclonal antibody. Whether you need optimized monoclonal alternatives, matched pairs, or custom reagent sourcing, we support your assay development pipeline.