Anti-Gelsolin Rabbit Monoclonal Antibody [KD Validated] for WB, IHC-P, ELISA - P06396

Core Product Specifications and Parameters

Biological Background: Gelsolin Function and Localization

• Gelsolin is an actin-modulating protein that severs, caps, and nucleates actin filaments in a calcium-dependent manner, regulating cytoskeletal remodeling.
• Gelsolin binds to the barbed ends of actin filaments, preventing monomer exchange (capping) and promoting filament nucleation. It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed (PubMed:19666512). Related references: PMID:19666512
• Gelsolin also plays a role in ciliogenesis, the process by which cells form primary cilia (PubMed:20393563). Related references: PMID:20393563
• The protein is localized in the cytoplasm and associated with the cytoskeleton; it can also be secreted.
• Gelsolin is expressed in phagocytic cells, platelets, fibroblasts, and both smooth and skeletal muscle cells.
• Alternative initiation and splicing produce multiple gelsolin isoforms, each with distinct N-terminal sequences. The protein undergoes post-translational modifications such as acetylation and phosphorylation.
• Genetic variants in GSN are associated with diseases like amyloidosis and corneal dystrophy.

Experimental Guidance and Technical Tips

• The immunogen spans almost the full-length human gelsolin protein (aa 28–782), suggesting the antibody may recognize multiple epitopes and provide robust detection across applications.
• For Western blot, anticipate a band at approximately 86 kDa. Validate specificity using Gelsolin-positive cell lines (e.g., HeLa, platelets, fibroblasts) and consider knock-down (KD) controls.
• For IHC-P, optimize antigen retrieval and test on tissues with known high expression, such as smooth muscle or skin.
• In ELISA, pair with a suitable detection antibody after direct coating of recombinant gelsolin or use in sandwich formats if epitopes are compatible.
• Although the antibody is KD validated, performance in each assay should be confirmed in the relevant experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with streamlined access to validated IVD raw materials, technical services, and consulting—from concept to clinic. This anti-Gelsolin antibody supports actin cytoskeleton and cell biology research, and CamelBio can additionally supply matched antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets, ensuring a complete procurement solution for your assay development needs.