Anti-Gelsolin Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P06396

Core Product Specifications and Parameters

Biological Background: Gelsolin Function and Localization

• Gelsolin is a calcium-regulated, actin-modulating protein that binds to the barbed ends of actin filaments, capping them to prevent monomer exchange, while also promoting nucleation and severing of filaments (PubMed:19666512). Related references: PMID:19666512

• It plays a role in ciliogenesis, contributing to cilium biogenesis and degradation (PubMed:20393563). Related references: PMID:20393563

• The protein is predominantly localized in the cytoplasm and cytoskeleton, and can also be secreted, indicating both intracellular and extracellular functions.

• Tissue expression is found in phagocytic cells, platelets, fibroblasts, non-muscle cells, and smooth and skeletal muscle cells.

• Alternative splicing generates multiple isoforms; the full-length canonical form has a molecular weight of approximately 86 kDa, consistent with the observed protein weight.

• Post-translational modifications include acetylation, phosphorylation, and disulfide bond formation, which may modulate its actin-binding and regulatory activities.

• Mutations in the GSN gene are associated with amyloidosis and corneal dystrophy, and gelsolin itself can form amyloid fibrils under pathological conditions.

Experimental Guidance and Technical Tips

• The antibody is a rabbit polyclonal raised against a recombinant fragment encompassing amino acids 282-438 of human gelsolin; it may detect multiple isoforms or processing variants depending on the sample.
• For Western blot (WB), expect a major band near 86 kDa; additional bands may appear due to isoform diversity or proteolytic fragments.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), optimize antigen retrieval and fixation conditions for your specific tissue or cell type.
• Cross-reactivity is reported with mouse and rat gelsolin, making it suitable for rodent model studies; however, confirmatory validation in the target species is recommended.
• For ELISA applications, use as per standard protocols; titration of the antibody is advisable to achieve optimal signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, providing validated antibody pairs, optimized monoclonal and polyclonal antibodies like this gelsolin antibody, bulk ancillary reagents, and rare target raw-material sourcing. With expertise spanning from concept to clinic, we support diagnostic manufacturers and researchers in developing robust assays for actin cytoskeleton-related biomarkers. Let CamelBio streamline your supply chain with reliable, high-quality reagents and technical consulting tailored to your project needs.