Anti-Gelsolin Monoclonal Antibody for WB, IHC-P, ELISA - P06396

Core Product Specifications and Parameters

Biological Background: Gelsolin Function and Localization

• Gelsolin is a calcium-regulated actin-modulating protein that caps the barbed ends of actin filaments, severs pre-existing filaments, and promotes actin nucleation. Related references: PMID:19666512
• It plays a role in ciliogenesis, contributing to the assembly and maintenance of primary cilia. Related references: PMID:20393563
• Alternative splicing generates multiple isoforms, including a secreted plasma form (Brevin) that contains an additional N-terminal signal peptide.
• Gelsolin localizes to the cytoplasm and cytoskeleton, but the secreted isoform is found extracellularly; it is highly expressed in phagocytic cells, platelets, fibroblasts, and muscle cells.
• The protein consists of six gelsolin-like repeats and undergoes post-translational modifications such as phosphorylation and acetylation.
• Mutations in GSN are associated with familial amyloidosis and corneal dystrophy, with the protein found in amyloid plaques.

Experimental Guidance and Technical Tips

• The immunogen targets a conserved region (aa 400–500) within the gelsolin repeat domain, likely recognizing multiple human isoforms and possibly the mouse ortholog.
• For Western blot, expect a band near 86 kDa in human and mouse cell/tissue lysates; consider using appropriate loading controls.
• IHC-P may require antigen retrieval; validate staining in known positive tissues (e.g., platelets, muscle) and include isotype controls.
• ELISA can be used for protein or peptide detection; confirm linear epitope specificity and cross-reactivity with mouse Gelsolin.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your integrated partner for IVD raw-material procurement, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents to support your Gelsolin-related research and diagnostic development. Our streamlined supply chain bridges the gap from concept to clinic, ensuring reliable access to rare and routine targets for cytoskeleton and amyloidosis studies.