Anti-GDI2 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P50395

Core Product Specifications and Parameters

Biological Background: GDI2 (Rab GDP dissociation inhibitor beta) Function and Localization

• GDI2 is a ubiquitously expressed member of the Rab GDP dissociation inhibitor family that regulates intracellular membrane trafficking by maintaining Rab GTPases in their inactive GDP-bound state. Related references: PMID:25860027
• It plays a critical role in preventing GDP to GTP exchange on most Rab proteins, thereby controlling vesicle budding, transport, and fusion events. Related references: PMID:25860027
• GDI2 negatively regulates ciliogenesis by inhibiting RAB8A activity, thus controlling protein transport to the cilium. Related references: PMID:25860027
• The protein localizes primarily to the cytoplasm and associates with membranes, including the Golgi apparatus and trans-Golgi network.
• Post-translational modifications such as acetylation and phosphorylation may modulate its function and interactions.
• Alternative splicing generates multiple isoforms, potentially diversifying its regulatory roles.
• As a reference proteome member, GDI2 is widely used in proteomics identification studies.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 296–445, covering the C-terminal region; consider this when designing truncation or domain-specific assays.
• For Western blotting, validate the antibody in your model system and consider optimizing protein loading and transfer conditions due to the ~51 kDa size.
• In immunofluorescence, include appropriate controls and consider fixation/permeabilization methods to preserve target localization.
• ELISA performance may be influenced by coating antigen purity and conformation; titrate the antibody for optimal signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw material procurement by offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents for targets like GDI2—critical in intracellular trafficking and ciliogenesis research. As a one-stop partner from concept to clinic, we also source rare-target raw materials, helping diagnostic manufacturers and labs accelerate development.